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  • CD154 (CD40 Ligand) Antibodies

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CD154 Monoclonal Antibody (MR1), PE

View all (84) CD154 (CD40 Ligand) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite CD154 Monoclonal Antibody (MR1), PE

  • Antibody Testing Data (3)
CD154 Antibody in Flow Cytometry (Flow)
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CD154 Antibody in Flow Cytometry (Flow)
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FIGURE: 1 / 3

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CD154 Antibody (PE-65129-100UG) in Flow

1X10^6 PMA (50 ng/mL) and ionomycin (1 µg/mL)-stimulated Mouse lymphoid cells (6 hours) were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 0.06 µg PE Anti-Mouse CD154 (Product # PE-65129-100UG), Clone: MR1). Cells were not fixed. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CD154 Antibody in Flow Cytometry (Flow)
CD154 Antibody in Flow Cytometry (Flow)
CD154 Antibody in Flow Cytometry (Flow)
CD154 Monoclonal Antibody (MR1), PE

Product Details

PE-65129-100UG

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

MR1

Conjugate

PE PE PE
  • CoraLite Plus 488
  • CoraLite Plus 647
  • FITC
  • Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Wet ice

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: CD154; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

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Gene Aliases: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP

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UniProt ID: (Mouse) P27548

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Entrez Gene ID: (Mouse) 21947

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Function(s)
cytokine activity tumor necrosis factor receptor binding CD40 receptor binding
Process(es)
positive regulation of immune response to tumor cell inflammatory response immune response signal transduction platelet activation B cell differentiation positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production B cell proliferation negative regulation of apoptotic process isotype switching positive regulation of MHC class II biosynthetic process immunoglobulin secretion regulation of immunoglobulin secretion dendritic cell differentiation positive regulation of endothelial cell apoptotic process
It has to be done as per old AB suggested Products section.
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