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Invitrogen
CCL3 Monoclonal Antibody, PeproTech®
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FIGURE: 1 / 3
CCL3 Antibody (500-M74-500UG) in WB
Product Details
500-M74-500UG
Species Reactivity
Published species
Host/Isotype
Class
Type
Immunogen
Conjugate
Form
Purification
Storage buffer
Contains
Storage conditions
Shipping conditions
RRID
Product Specific Information
AA Sequence of recombinant protein: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA.
Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MIP-1alpha (CCL3). Anti-Human MIP-1alpha (CCL3)-specific antibody was purified from cell culture by Protein G affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 2000 pg/mL of Recombinant Human MIP-1alpha (CCL3) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MIP-1alpha (CCL3) (500-P38GBT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL.
Western Blot: To detect Human MIP-1alpha (CCL3) by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-1alpha (CCL3) is 1.0-2.0 ng/lane, under non-reducing conditions.
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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