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  • Primary Antibodies ›
  • CCL23 Antibodies

Invitrogen

CCL23 Polyclonal Antibody, PeproTech®

1 Reference
View all (15) CCL23 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite CCL23 Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
CCL23 Antibody in ELISA (ELISA)
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CCL23 Antibody in ELISA (ELISA)
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CCL23 Antibody (500-P124-1MG) in ELISA

Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL23 Polyclonal Antibody (Product # 500-P124-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL23 Polyclonal Antibody, Biotin (Product # 500-P124BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CCL23 Antibody in Western Blot (WB)
CCL23 Antibody in Western Blot (WB)
CCL23 Antibody in ELISA (ELISA)
CCL23 Polyclonal Antibody, PeproTech®

Product Details

500-P124-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MIP-3 (CCL23)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929348

Product Specific Information

AA Sequence of recombinant protein: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23). Anti-Human MIP-3 (CCL23)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3 (CCL23) matrix.

Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3 (CCL23) (500-P124Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23).

Western Blot: To detect Human MIP-3 (CCL23) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 23; C6 beta-chemokine; chemokine (C-C motif) ligand 23; Ck beta 8-1; CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; MIP-3CK-BETA-8; MPIF-1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23

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Gene Aliases: CCL23; CK-BETA-8; Ckb-8; Ckb-8-1; CKb8; hmrp-2a; MIP-3; MIP3; MPIF-1; MPIF1; SCYA23

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UniProt ID: (Human) P55773

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Entrez Gene ID: (Human) 6368

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Function(s)
chemokine activity heparin binding CCR1 chemokine receptor binding
Process(es)
monocyte chemotaxis cellular calcium ion homeostasis chemotaxis inflammatory response immune response signal transduction G-protein coupled receptor signaling pathway cell-cell signaling negative regulation of cell proliferation neutrophil chemotaxis positive regulation of GTPase activity lymphocyte chemotaxis positive regulation of inflammatory response chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor negative regulation of C-C chemokine binding
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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