Invitrogen

CACNB2 Monoclonal Antibody (N8b/1), PE

Name: CACNB2 Monoclonal Antibody (N8b\/1), PE
Brand: Invitrogen
SKU: MA5-45453

View all (30) CACNB2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

CACNB2 Antibody in Immunocytochemistry (ICC/IF)

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CACNB2 Antibody (MA5-45453) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Cav beta 2 monoclonal antibody (Product # MA5-45453) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 antibody (D) Compo... View More

CACNB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image CACNB2 Monoclonal Antibody (N8b/1), PE

Product Details

MA5-45453

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

Assay-dependent

Antibody Microarray (AM)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N8b/1

Immunogen

Synthetic peptide amino acids 189-205 of rat CavBeta2

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Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2931907

Product Specific Information

1 µg/mL of MA5-45453 was sufficient for detection of Cav beta2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody|Detects approximately 78 kDa. No cross reactivity against Cav beta 1, Cav beta 3, Cav beta 4.

Target Information

The expression of multiple classes of voltage dependent calcium channels (VDCCs) allows neurons to tailor calcium signaling to functionally discrete cellular regions. Although N-Type VDCCs exist before birth which is consistent with a role in migration, most N-Type VDCCs subunit expression is postnatal and is important in the genesis of synaptic transmission in discrete hippocampal fields.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CAB1; CAB2; CACNLB1; Calcium channel voltage-dependent subunit beta 2; calcium channel, voltage-dependent, beta 2 subunit; CCHLB1; Lambert-Eaton myasthenic syndrome antigen B; MGC138502; MGC138504; MGC41896; myasthenic (Lambert-Eaton) syndrome antigen B; MYSB; Voltage-dependent L-type calcium channel subunit beta-2; voltage-gated calcium channel beta2 subunit transcript variant X13

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Gene Aliases: AW060387; CAB2; CACNB2; CACNLB2; CAVB2; Cavbeta2; Cchb2; MYSB

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UniProt ID: (Human) Q08289, (Mouse) Q8CC27, (Rat) Q8VGC3

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Entrez Gene ID: (Human) 783, (Mouse) 12296, (Rat) 116600

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Function(s)

voltage-gated calcium channel activity calcium channel regulator activity calcium channel activity protein binding high voltage-gated calcium channel activity protein kinase binding protein domain specific binding actin filament binding phosphoprotein binding voltage-gated calcium channel activity involved in cardiac muscle cell action potential voltage-gated calcium channel activity involved in AV node cell action potential voltage-gated ion channel activity

Process(es)

chemical synaptic transmission neuromuscular junction development visual perception positive regulation of calcium ion transport cardiac conduction calcium ion import calcium ion transmembrane transport membrane depolarization during AV node cell action potential regulation of heart rate by cardiac conduction establishment of protein localization to plasma membrane membrane depolarization during atrial cardiac muscle cell action potential regulation of voltage-gated calcium channel activity positive regulation of high voltage-gated calcium channel activity positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel transport ion transport calcium ion transport regulation of ion transmembrane transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CACNB2 Monoclonal Antibody (N8b/1), PE

CACNB2 Antibody (MA5-45453) in ICC/IF

Product Details

MA5-45453

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Antibody Microarray (AM)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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