Invitrogen

BRD3 Polyclonal Antibody

Name: BRD3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-40885

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
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FIGURE: 1 / 8

BRD3 Antibody (PA5-40885) in WB

Western blot was performed using Anti-BRD3 Polyclonal Antibody (Product # PA5-40885) and two isoforms at 60.95 kDa and 100 kDa corresponding to BRD3 were observed along with few non specific bands in certain cell lines and tissues tested. Whole cell extracts (30 µg lysate) of NTERA-2 cl.D1 (Lane 1), A549 (Lane 2), SH-SY5Y (Lane 3), HeLa (Lane 4), IMR-32 (Lane 5), NIH/3T3 (Lane 6), Mouse Kidney (Lane 7), Rat Kidney (Lane 8) and Mouse Lung (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved ... View More

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BRD3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells lines tested owing to their inherent genetic constitution. Relative expression of BRD3 was observed in, NTERA-2 cl.D1 (Lane 1) High expression, A549 (Lane 2) Low expression, SH-SY5Y (Lane 3) High expression, HeLa (Lane 4) Low expression, IMR-32 (Lane 5) High expression, NIH/3T3 (Lane 6) Low expression, as reported for BRD3 expression using BRD3 Polyclonal Antibody (Product # PA5-40885) in Western Blot. {RE}

Product Details

PA5-40885

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

- -

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human BRD3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2607784

Product Specific Information

Peptide sequence: SGKKQAAKSK EELAQEKKKE LEKRLQDVSG QLSSSKKPAR KEKPGSAPSG

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%

Target Information

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bromodomain-containing 3; Bromodomain-containing protein 3; bromodomain-containing protein 3 short isoform; RING3-like protein

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Gene Aliases: BRD3; KIAA0043; ORFX; RING3L

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UniProt ID: (Human) Q15059

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Entrez Gene ID: (Human) 8019

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Function(s)

chromatin binding protein binding lysine-acetylated histone binding

Process(es)

transcription, DNA-templated regulation of transcription from RNA polymerase II promoter covalent chromatin modification

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BRD3 Polyclonal Antibody

BRD3 Antibody (PA5-40885) in WB

Product Details

PA5-40885

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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