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  • Primary Antibodies ›
  • BRD3 Antibodies

Invitrogen

BRD3 Polyclonal Antibody

View all (24) BRD3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite BRD3 Polyclonal Antibody

  • Antibody Testing Data (7)
BRD3 Antibody in Immunocytochemistry (ICC/IF)
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BRD3 Antibody in Immunocytochemistry (ICC/IF)
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BRD3 Antibody (PA5-30263) in ICC/IF

Immunofluorescence analysis of BRD3 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BRD3 Polyclonal Antibody (Product # PA5-30263) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Prod... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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BRD3 Antibody in Immunocytochemistry (ICC/IF)
BRD3 Antibody in Immunocytochemistry (ICC/IF)
BRD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
BRD3 Antibody in Western Blot (WB)
BRD3 Antibody in Western Blot (WB)
BRD3 Antibody in Western Blot (WB)
BRD3 Antibody in ChIP Assay (ChIP)
BRD3 Polyclonal Antibody

Product Details

PA5-30263

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ChIP assay (ChIP)

2.5 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BRD3. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2547737

Product Specific Information

Recommended positive controls: HeLa nucleus, mouse Liver.

Predicted reactivity: Rat (93%), Rhesus Monkey (98%), Bovine (81%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bromodomain-containing 3; Bromodomain-containing FSH-like protein FSRG2; Bromodomain-containing protein 3; bromodomain-containing protein 3 short isoform; RING3-like protein

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Gene Aliases: 2410084F24Rik; AW060456; BRD3; Fsrg2; KIAA0043; ORFX; RING3L; RINGL3

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UniProt ID: (Human) Q15059, (Mouse) Q8K2F0

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Entrez Gene ID: (Human) 8019, (Mouse) 67382

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Function(s)
chromatin binding protein binding lysine-acetylated histone binding
Process(es)
transcription, DNA-templated regulation of transcription from RNA polymerase II promoter covalent chromatin modification regulation of transcription, DNA-templated chromatin modification
It has to be done as per old AB suggested Products section.
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