Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Primary Antibodies ›
  • BIK Antibodies

Invitrogen

BIK Polyclonal Antibody

Advanced Verification
1 Published Figure
1 Reference
View all (14) BIK antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite BIK Polyclonal Antibody

  • Antibody Testing Data (4)
  • Published Figures (1)
  • Advanced Verification (2)
BIK Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
BIK Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 7

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

BIK Antibody (PA5-20249) in ICC/IF

Immunofluorescence analysis of BIK was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BIK Polyclonal Antibody (Product # PA5-20249) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

BIK Antibody in Immunocytochemistry (ICC/IF)
BIK Antibody in Immunocytochemistry (ICC/IF)
BIK Antibody in Immunocytochemistry (ICC/IF)
BIK Antibody in Western Blot (WB)
BIK Antibody in Western Blot (WB)
BIK Antibody
BIK Antibody
BIK Polyclonal Antibody

Product Details

PA5-20249

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid peptide from near the amino terminus of human BIK.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153271

Product Specific Information

A suggested positive control is Jurkat cell lysate.

PA5-20249 can be used with blocking peptide PEP-0371.

Target Information

The Bik protein is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apoptosis inducer NBK; apoptosis-inducing NBK; BBC1; Bcl-2-interacting killer; BCL2-interacting killer (apoptosis-inducing); bhikhari; Bik-like killer protein; BIP 1; BIP1; BP 4; BP4; cb 60; natural born killer

View more View less

Gene Aliases: BIK; Biklk; BIP1; Blk; BP4; NBK

View more View less

UniProt ID: (Human) Q13323, (Mouse) O70337

View more View less

Entrez Gene ID: (Human) 638, (Mouse) 12124

View more View less

Function(s)
protein binding protein heterodimerization activity BH domain binding
Process(es)
apoptotic process spermatogenesis male gonad development apoptotic mitochondrial changes positive regulation of protein homooligomerization positive regulation of release of cytochrome c from mitochondria
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-649bfd9c6b-jlsz7:80/100.66.128.142:80.
git-commit: 6001d82975f0049f9efc09ec70b1b2d89229eeb3
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0