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  • Primary Antibodies ›
  • BHLHB5 Antibodies

Invitrogen

BHLHB5 Polyclonal Antibody

View all (8) BHLHB5 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite BHLHB5 Polyclonal Antibody

  • Antibody Testing Data (3)
BHLHB5 Antibody in Immunocytochemistry (ICC/IF)
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BHLHB5 Antibody in Immunocytochemistry (ICC/IF)
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BHLHB5 Antibody (PA5-100405) in ICC/IF

Immunocytochemistry analysis of BHLHB5 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with BHLHB5 polyclonal antibody (Product # PA5-100405) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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BHLHB5 Antibody in Immunocytochemistry (ICC/IF)
BHLHB5 Antibody in Western Blot (WB)
BHLHB5 Antibody in Western Blot (WB)
BHLHB5 Polyclonal Antibody

Product Details

PA5-100405

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human BHLHE22(Accession Q8NFJ8), corresponding to amino acid residues L119-A169.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849917

Product Specific Information

Antibody detects endogenous levels of total BHLHE22.

Target Information

BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: basic helix-loop-helix (bHLH) gene, class B, beta3; basic helix-loop-helix domain containing, class B, 5; basic helix-loop-helix domain containing, class B5; basic helix-loop-helix family, member e22; bHLHb5; bHLHe22; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; Protein BETA3; trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein

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Gene Aliases: Beta3; BHLHB5; BHLHE22; CAGL85; TNRC20

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UniProt ID: (Human) Q8NFJ8, (Mouse) Q8C6A8

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Entrez Gene ID: (Human) 27319, (Mouse) 59058, (Rat) 365748

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding protein dimerization activity chromatin binding protein binding protein homodimerization activity
Process(es)
transcription, DNA-templated regulation of transcription from RNA polymerase II promoter neurogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter corpus callosum morphogenesis cerebral cortex regionalization central nervous system projection neuron axonogenesis corticospinal tract morphogenesis anterior commissure morphogenesis neuron differentiation negative regulation of transcription, DNA-templated retinal bipolar neuron differentiation retina morphogenesis in camera-type eye
It has to be done as per old AB suggested Products section.
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