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  • BCAT1 Antibodies

Invitrogen

BCAT1 Polyclonal Antibody

View all (14) BCAT1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite BCAT1 Polyclonal Antibody

  • Antibody Testing Data (3)
BCAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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BCAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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BCAT1 Antibody (PA5-78859) in IHC (P)

Immunohistochemical analysis of BCAT1 in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-BCAT1 antibody (Product # PA5-78859) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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BCAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
BCAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
BCAT1 Antibody in Western Blot (WB)
BCAT1 Polyclonal Antibody

Product Details

PA5-78859

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1 (360-375aa KLASRILSKLTDIQYGR).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745975

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Raji whole cell, human MOLT-4 whole cell. IHC: human ovarian adenoma tissue, human pancreatic cancer tissue.

Target Information

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BCAT(c); branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1, cytosolic; Branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18

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Gene Aliases: BCAT1; BCATC; BCT1; ECA39; MECA39; PNAS121; PP18

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UniProt ID: (Human) P54687

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Entrez Gene ID: (Human) 586

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Function(s)
branched-chain-amino-acid transaminase activity pyridoxal phosphate binding identical protein binding L-leucine transaminase activity L-valine transaminase activity L-isoleucine transaminase activity
Process(es)
G1/S transition of mitotic cell cycle aspartate biosynthetic process cell proliferation branched-chain amino acid biosynthetic process branched-chain amino acid catabolic process leucine biosynthetic process valine biosynthetic process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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