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  • Primary Antibodies ›
  • BAFF (BLyS) Antibodies

Invitrogen

BAFF Polyclonal Antibody

View all (33) BAFF (BLyS) antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite BAFF Polyclonal Antibody

  • Antibody Testing Data (2)
BAFF Antibody in Western Blot (WB)
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BAFF Antibody in Western Blot (WB)
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BAFF Antibody (PA5-81086) in WB

Western Blot using BAFF Polyclonal Antibody (Product # PA5-81086) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 36 kDa. (We are unsure of the identity of these extra bands). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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BAFF Antibody in Western Blot (WB)
BAFF Antibody in Immunoprecipitation (IP)
BAFF Polyclonal Antibody

Product Details

PA5-81086

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:5,000-1:10,000
-

Immunoprecipitation (IP)

1-4 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human BAFF/TNFSF13B protein (Ala134-Leu285)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2788333

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human BAFF/TNFSF13B.

Target Information

BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLyS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; TALL-1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF- and APOL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor-like protein ZTNF4

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Gene Aliases: BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF13B; TNFSF20; TNLG7A; UNQ401/PRO738; ZTNF4

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UniProt ID: (Human) Q9Y275

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Entrez Gene ID: (Human) 10673

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Function(s)
receptor binding cytokine activity tumor necrosis factor receptor binding protein binding
Process(es)
immune response signal transduction cell proliferation positive regulation of cell proliferation positive regulation of B cell proliferation tumor necrosis factor-mediated signaling pathway
It has to be done as per old AB suggested Products section.
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