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  • Apolipoprotein H Antibodies

Invitrogen

Apolipoprotein H Recombinant Rabbit Monoclonal Antibody (003)

Advanced Verification
View all (37) Apolipoprotein H antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Apolipoprotein H Recombinant Rabbit Monoclonal Antibody (003)

  • Antibody Testing Data (3)
  • Advanced Verification (2)
Apolipoprotein H Antibody in Immunocytochemistry (ICC/IF)
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Apolipoprotein H Antibody in Immunocytochemistry (ICC/IF)
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Apolipoprotein H Antibody (MA5-29036) in ICC/IF

Immunofluorescence analysis of Beta-2-glycoprotein 1 was performed using 70% confluent log phase Hep G2, Hep G2 treated with PTI (1X for 4hrs) and PC-3 cells treated with PTI (1X for 4hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Apolipoprotein H... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Apolipoprotein H Antibody in Immunocytochemistry (ICC/IF)
Apolipoprotein H Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Apolipoprotein H Antibody in Western Blot (WB)
Apolipoprotein H Antibody
Apolipoprotein H Antibody
Apolipoprotein H Recombinant Rabbit Monoclonal Antibody (003)

Product Details

MA5-29036

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:200
-

ELISA (ELISA)

1:5,000-1:10,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

003

Immunogen

Recombinant Human APOH protein (Met1-Cys345)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784971

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human Apolipoprotein H/APOH.

Target Information

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2GPI; Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; Apo-H; Apolipoprotein H; apolipoprotein H (beta-2-glycoprotein I); B2GPI; beta(2)GPI; Beta-2-glycoprotein 1; Beta-2-glycoprotein I

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Gene Aliases: APOH; B2G1; B2GP1; BG

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UniProt ID: (Human) P02749

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Entrez Gene ID: (Human) 350

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Function(s)
glycoprotein binding protein binding phospholipid binding heparin binding lipid binding identical protein binding lipoprotein lipase activator activity
Process(es)
negative regulation of endothelial cell proliferation platelet degranulation triglyceride metabolic process aging blood coagulation, intrinsic pathway negative regulation of endothelial cell migration positive regulation of triglyceride catabolic process negative regulation of angiogenesis positive regulation of blood coagulation negative regulation of blood coagulation organ regeneration plasminogen activation negative regulation of myeloid cell apoptotic process response to triglyceride triglyceride transport negative regulation of smooth muscle cell apoptotic process positive regulation of lipoprotein lipase activity regulation of fibrinolysis negative regulation of fibrinolysis negative regulation of respiratory burst
It has to be done as per old AB suggested Products section.
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