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  • Primary Antibodies ›
  • Apolipoprotein H Antibodies

Invitrogen

Apolipoprotein H Polyclonal Antibody

Advanced Verification
View all (37) Apolipoprotein H antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Apolipoprotein H Polyclonal Antibody

  • Antibody Testing Data (2)
  • Advanced Verification (1)
Apolipoprotein H Antibody in Western Blot (WB)
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Apolipoprotein H Antibody in Western Blot (WB)
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Apolipoprotein H Antibody (PA5-88412) in WB

Western Blot was performed using Anti-Apolipoprotein H Polyclonal Antibody (Product # PA5-88412) and a 50 kDa band corresponding to Beta-2-glycoprotein 1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X for 4 hours) (Lane 2), PC-3 (Lane 3), PC-3 treated with PTI (1X for 4 hours) (Lane 4), BeWo (Lane 5), BeWo treated with PTI (1X for 4 hours) (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transf... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Apolipoprotein H Antibody in Western Blot (WB)
Apolipoprotein H Antibody in Western Blot (WB)
Apolipoprotein H Antibody
Apolipoprotein H Polyclonal Antibody

Product Details

PA5-88412

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-345 of human APOH (NP_0000332)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.81 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2804894

Product Specific Information

Immunogen sequence: RTCPKPDDLP FSTVVPLKTF YEPGEEITYS CKPGYVSRGG MRKFICPLTG LWPINTLKCT PRVCPFAGIL ENGAVRYTTF EYPNTISFSC NTGFYLNGAD SAKCTEEGKW SPELPVCAPI ICPPPSIPTF ATLRVYKPSA GNNSLYRDTA VFECLPQHAM FGNDTITCTT HGNWTKLPEC REVKCPFPSR PDNGFVNYPA KPTLYYKDKA TFGCHDGYSL DGPEEIECTK LGNWSAMPSC KASCKVPVKK ATVVYQGERV KIQEKFKNGM LHGDKVSFFC KNKEKKCSYT EDAQCIDGTI EVPKCFKEHS SLAFWKTDAS DVKPC; Positive Samples: 22Rv1

Target Information

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2GPI; Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; Apo-H; Apolipoprotein H; apolipoprotein H (beta-2-glycoprotein I); B2GPI; beta(2)GPI; Beta-2-glycoprotein 1; Beta-2-glycoprotein I

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Gene Aliases: APOH; B2G1; B2GP1; BG

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UniProt ID: (Human) P02749

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Entrez Gene ID: (Human) 350

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Function(s)
glycoprotein binding protein binding phospholipid binding heparin binding lipid binding identical protein binding lipoprotein lipase activator activity
Process(es)
negative regulation of endothelial cell proliferation platelet degranulation triglyceride metabolic process aging blood coagulation, intrinsic pathway negative regulation of endothelial cell migration positive regulation of triglyceride catabolic process negative regulation of angiogenesis positive regulation of blood coagulation negative regulation of blood coagulation organ regeneration plasminogen activation negative regulation of myeloid cell apoptotic process response to triglyceride triglyceride transport negative regulation of smooth muscle cell apoptotic process positive regulation of lipoprotein lipase activity regulation of fibrinolysis negative regulation of fibrinolysis negative regulation of respiratory burst
It has to be done as per old AB suggested Products section.
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