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  • Primary Antibodies ›
  • Annexin A4 Antibodies

Invitrogen

Annexin A4 Polyclonal Antibody

View all (23) Annexin A4 antibodies

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Datasheet
Protocols
Questions & Answers

Cite Annexin A4 Polyclonal Antibody

  • Antibody Testing Data (1)
Annexin A4 Antibody in Western Blot (WB)
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Annexin A4 Antibody in Western Blot (WB)
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Annexin A4 Antibody (PA5-92862) in WB

Western blot analysis of Annexin A4 in extracts of various cell lines. Samples were incubated with Annexin A4 Polyclonal antibody (Product # PA5-92862) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Annexin A4 Antibody in Western Blot (WB)
Annexin A4 Polyclonal Antibody

Product Details

PA5-92862

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 112-321 of human Annexin A4/Annexin IV (NP_0011441)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.56 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806521

Product Specific Information

Immunogen sequence: IEILASRTPE EIRRISQTYQ QQYGRSLEDD IRSDTSFMFQ RVLVSLSAGG RDEGNYLDDA LVRQDAQDLY EAGEKKWGTD EVKFLTVLCS RNRNHLLHVF DEYKRISQKD IEQSIKSETS GSFEDALLAI VKCMRNKSAY FAEKLYKSMK GLGTDDNTLI RVMVSRAEID MLDIRAHFKR LYGKSLYSFI KGDTSGDYRK VLLVLCGGDD; Positive Samples: BxPC-3, MCF7, A-549, SW620, HepG2, Mouse liver, Mouse kidney, Mouse lung, Rat kidney

Target Information

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 35-beta calcimedin; 36 kDa zymogen granule membrane-associated protein; Annexin A4; Annexin IV; annexin IV (placental anticoagulant protein II); Annexin-4; ANXA 4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; epididymis secretory protein Li 274; Lipocortin IV; P32.5; PAP-II; Placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; Protein II; ZAP 36/annexin IV; ZAP36

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Gene Aliases: AI265406; AIV; ANX4; ANXA4; AW106930; HEL-S-274; P32.5; PAP-II; PIG28; PP4-X; Xanx-4; ZAP36

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UniProt ID: (Human) P09525, (Mouse) P97429

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Entrez Gene ID: (Human) 307, (Rat) 79124, (Mouse) 11746

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Function(s)
phospholipase inhibitor activity calcium ion binding protein binding calcium-dependent phospholipid binding identical protein binding calcium-dependent protein binding NF-kappaB binding
Process(es)
regulation of transcription from RNA polymerase II promoter exocytosis epithelial cell differentiation negative regulation of NF-kappaB transcription factor activity negative regulation of interleukin-8 secretion kidney development Notch signaling pathway signal transduction negative regulation of apoptotic process negative regulation of catalytic activity
It has to be done as per old AB suggested Products section.
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