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  • Ankyrin G Antibodies

Invitrogen

Ankyrin G Monoclonal Antibody (N106/20), PerCP

View all (14) Ankyrin G antibodies

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Datasheet
Protocols
Questions & Answers

Cite Ankyrin G Monoclonal Antibody (N106/20), PerCP

  • Antibody Testing Data (3)
Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
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Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
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Ankyrin G Antibody (MA5-45542) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Ankyrin G monoclonal antibody (Product # MA5-45542) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 fo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
Ankyrin G Antibody in Western Blot (WB)

Product Details

MA5-45542

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N106/20

Immunogen

Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats (aa 3377-4377).

Conjugate

PerCP PerCP PerCP
  • APC 
  • FITC
  • Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931996

Product Specific Information

1 µg/mL of MA5-45542 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects >approximately 200kDa.

This antibody was formerly sold as clone S106-20.

Target Information

Ankyrin-G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin-G is also found at sites of epithelial cell-cell contact and in the intracellular membranes of some cells. Ankyrin-G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin-G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ANK-3; ankyrin 3 (G); ankyrin 3, epithelial; Ankyrin-3; Ankyrin-G; brain-specific ankyrin-G

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Gene Aliases: 2900054D09Rik; AI314020; ANK-3; ANK3; AnkG; Ankyrin-3; ANKYRIN-G; MRT37

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UniProt ID: (Human) Q12955, (Rat) O70511, (Mouse) G5E8K5

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Entrez Gene ID: (Human) 288, (Rat) 361833, (Mouse) 11735

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Function(s)
structural constituent of cytoskeleton protein binding cytoskeletal protein binding cytoskeletal adaptor activity spectrin binding protein binding, bridging ion channel binding cadherin binding
Process(es)
mitotic cytokinesis plasma membrane organization signal transduction axonogenesis axon guidance neuromuscular junction development positive regulation of gene expression positive regulation of cell communication by electrical coupling positive regulation of sodium ion transport magnesium ion homeostasis neuronal action potential positive regulation of homotypic cell-cell adhesion Golgi to plasma membrane protein transport regulation of potassium ion transport establishment of protein localization positive regulation of membrane potential synapse organization cellular response to magnesium ion membrane assembly protein localization to plasma membrane maintenance of protein location in plasma membrane protein targeting to plasma membrane positive regulation of protein targeting to membrane positive regulation of membrane depolarization during cardiac muscle cell action potential negative regulation of delayed rectifier potassium channel activity positive regulation of sodium ion transmembrane transporter activity positive regulation of cation channel activity ER to Golgi vesicle-mediated transport cytoskeletal anchoring at plasma membrane positive regulation of action potential
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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