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  • Amnionless Antibodies

Invitrogen

Amnionless Polyclonal Antibody

View all (4) Amnionless antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Amnionless Polyclonal Antibody

  • Antibody Testing Data (2)
Amnionless Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Amnionless Antibody in Immunohistochemistry (Frozen) (IHC (F))
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FIGURE: 1 / 2

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Amnionless Antibody (PA5-47903) in IHC (F)

Immunohistochemical analysis of Amnionless in perfusion fixed frozen sections of mouse kidney. Samples were incubated with Amnionless polyclonal antibody (Product # PA5-47903) using a dilution of 15 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubules. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Amnionless Antibody in Immunohistochemistry (Frozen) (IHC (F))
Amnionless Antibody in Western Blot (WB)
Amnionless Polyclonal Antibody

Product Details

PA5-47903

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHO-derived recombinant mouse Amnionless Ala20-Gly362
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605809

Product Specific Information

In direct ELISAs, approximately 10% cross-reactivity with recombinant human Amnionless is observed.

Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Target Information

The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Protein amnionless; type I transmembrane protein

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Gene Aliases: 5033428N14Rik; Amn; AV002116

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UniProt ID: (Mouse) Q99JB7

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Entrez Gene ID: (Mouse) 93835

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Function(s)
receptor binding
Process(es)
receptor-mediated endocytosis multicellular organism development excretion protein localization cobalamin transport Golgi to plasma membrane protein transport
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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