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Rockland

Alkaline Phosphatase Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Alkaline Phosphatase Polyclonal Antibody

Alkaline Phosphatase Polyclonal Antibody

Product Details

200-1135-0100

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:4,000-1:20,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Bovine

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Alkaline Phosphatase [Calf Intestine]
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase tissue non-specific isoform; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney isozyme; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; TNSALP

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Gene Aliases: ALPL; BOS_2299; TNS-AP

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UniProt ID: (Bovine) P09487

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Entrez Gene ID: (Bovine) 280994

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Function(s)
alkaline phosphatase activity pyrophosphatase activity metal ion binding
Process(es)
endochondral ossification developmental process involved in reproduction response to vitamin D response to antibiotic cellular response to organic cyclic compound
It has to be done as per old AB suggested Products section.
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