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Rockland

Alkaline Phosphatase Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Cite Alkaline Phosphatase Polyclonal Antibody

  • Antibody Testing Data (1)
Alkaline Phosphatase Antibody in Western Blot (WB)
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Alkaline Phosphatase Antibody in Western Blot (WB)
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Alkaline Phosphatase Antibody (100-401-142) in WB

Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 Dalton enzyme in cellular extracts. Approximately 10 µg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Alkaline Phosphatase Antibody in Western Blot (WB)
Alkaline Phosphatase Polyclonal Antibody

Product Details

100-401-142

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:5,000-1:20,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Alkaline Phosphatase [Human Intestine]
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

85 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue-nonspecific ALP

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Gene Aliases: ALPL; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP

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UniProt ID: (Human) P05186

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Entrez Gene ID: (Human) 249

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Function(s)
alkaline phosphatase activity protein binding pyrophosphatase activity metal ion binding
Process(es)
skeletal system development osteoblast differentiation endochondral ossification developmental process involved in reproduction dephosphorylation response to lipopolysaccharide response to vitamin D response to antibiotic response to glucocorticoid cellular response to organic cyclic compound cementum mineralization
It has to be done as per old AB suggested Products section.
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