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  • Afadin Antibodies

Invitrogen

Afadin Polyclonal Antibody

View all (12) Afadin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Afadin Polyclonal Antibody

  • Antibody Testing Data (5)
Afadin Antibody in Immunocytochemistry (ICC/IF)
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Afadin Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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Afadin Antibody (PA5-83131) in ICC/IF

Immunofluorecent analysis of Afadin in human cell line U-2 OS using Afadin Polyclonal Antibody (Product # PA5-83131). Staining shows localization to nuclear speckles, plasma membrane and cell junctions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Afadin Antibody in Immunocytochemistry (ICC/IF)
Afadin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Afadin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Afadin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Afadin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Afadin Polyclonal Antibody

Product Details

PA5-83131

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500
-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human AFDN. Recombinant protein control fragment (Product #RP-94823).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2790287

Product Specific Information

Immunogen sequence: KPEKPSTLQR PQETVIRELQ PQQQPRTIER RDLQYITVSK EELSSGDSLS PDPWKRDAKE KLEKQQQMHI VDMLSKEIQE LQSKPDRSAE ESDRLRKLML EWQ

Target Information

AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Afadin; Afadin adherens junction formation factor; ALL1-fused gene from chromosome 6 protein; FLJ34371; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; Protein AF-6; RP3-431P23.3; RP3-470B24.4

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Gene Aliases: AF6; AFDN; MLL-AF6; MLLT4

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UniProt ID: (Human) P55196

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Entrez Gene ID: (Human) 4301

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Function(s)
protein binding protein C-terminus binding Ras GTPase binding cadherin binding involved in cell-cell adhesion
Process(es)
cell adhesion signal transduction cell-cell signaling adherens junction organization positive regulation of GTPase activity establishment of endothelial intestinal barrier cell-cell adhesion
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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