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  • Aconitase 2 Antibodies

Invitrogen

Aconitase 2 Recombinant Rabbit Monoclonal Antibody (JE40-83)

Advanced Verification
View all (31) Aconitase 2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Aconitase 2 Recombinant Rabbit Monoclonal Antibody (JE40-83)

  • Antibody Testing Data (10)
  • Advanced Verification (2)
Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
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Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
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Aconitase 2 Antibody (MA5-34820) in ICC/IF

Immunofluorescence analysis of Aco2; Aconitase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Aconitase 2 Recombinant Rabbit Monoclonal Antibody (JE40-83) (Product # MA5-34820) at 1:200 dilution in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody
Aconitase 2 Antibody
Aconitase 2 Recombinant Rabbit Monoclonal Antibody (JE40-83)

Product Details

MA5-34820

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Immunoprecipitation (IP)

1:10-1:50
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE40-83

Immunogen

Recombinant protein within Human Aconitase 2 aa 1-190
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848728

Product Specific Information

Positive Control: SiHa, HL-60, rat epididymis tissue, human prostate cancer tissue, human kidney tissue, human esophagus tissue, mouse testis tissue.

Target Information

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aconitase; aconitase 2, mitochondrial; Aconitate hydratase, mitochondrial; Citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; MGC20605; MGC33908; mitochondrial aconitase; RP3-347H13.8

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Gene Aliases: Aco-2; ACO2; Aco3; ACONM; D10Wsu183e; HEL-S-284; ICRD; OCA8; OPA9

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UniProt ID: (Human) Q99798, (Mouse) Q99KI0

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Entrez Gene ID: (Human) 50, (Mouse) 11429

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Function(s)
aconitate hydratase activity iron ion binding 3 iron, 4 sulfur cluster binding 4 iron, 4 sulfur cluster binding lyase activity metal ion binding iron-sulfur cluster binding
Process(es)
tricarboxylic acid cycle citrate metabolic process isocitrate metabolic process metabolic process generation of precursor metabolites and energy
It has to be done as per old AB suggested Products section.
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