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  • Primary Antibodies ›
  • Aconitase 2 Antibodies

Invitrogen

Aconitase 2 Polyclonal Antibody

Advanced Verification
View all (31) Aconitase 2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Aconitase 2 Polyclonal Antibody

  • Antibody Testing Data (13)
  • Advanced Verification (2)
Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
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Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
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Aconitase 2 Antibody (PA5-29960) in ICC/IF

Immunofluorescence analysis of Aco2; Aconitase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Aconitase 2 Polyclonal Antibody (Product # PA5-29960) at 1:200 dilution in 0.1% BSA, incubated at 4 deg... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
Aconitase 2 Antibody in Immunocytochemistry (ICC/IF)
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Western Blot (WB)
Aconitase 2 Antibody in Immunoprecipitation (IP)
Aconitase 2 Antibody
Aconitase 2 Antibody

Product Details

PA5-29960

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:20,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2547434

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, JurKat, Mouse brain, rat brain.

Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (91%), Xenopus laevis (94%), Pig (98%), Chicken (95%), Rhesus Monkey (98%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aconitase; aconitase 2, mitochondrial; Aconitate hydratase, mitochondrial; Citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; MGC20605; MGC33908; mitochondrial aconitase; mitochondrial aconitase (nuclear aco2 gene); RP3-347H13.8

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Gene Aliases: Aco-2; ACO2; Aco3; ACONM; D10Wsu183e; HEL-S-284; ICRD; OCA8; OPA9; RCJMB04_1a14

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UniProt ID: (Human) Q99798, (Rat) Q9ER34, (Mouse) Q99KI0

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Entrez Gene ID: (Chicken) 374009, (Human) 50, (Rat) 79250, (Mouse) 11429

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Function(s)
aconitate hydratase activity iron ion binding 3 iron, 4 sulfur cluster binding 4 iron, 4 sulfur cluster binding protein binding lyase activity metal ion binding iron-sulfur cluster binding
Process(es)
tricarboxylic acid cycle citrate metabolic process isocitrate metabolic process metabolic process generation of precursor metabolites and energy
It has to be done as per old AB suggested Products section.
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