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OriGene

ATP5I Monoclonal Antibody (OTI1G4), TrueMAB™

View all (13) ATP5I antibodies

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Datasheet
Protocols
Questions & Answers

Cite ATP5I Monoclonal Antibody (OTI1G4), TrueMAB™

  • Antibody Testing Data (3)
ATP5I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ATP5I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ATP5I Antibody (CF811806) in IHC (P)

Immunohistochemical staining of paraffin-embedded human appendix tissue within the normal limits using anti-ATP5I mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811806)(1:500). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP5I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5I Antibody in Western Blot (WB)
ATP5I Monoclonal Antibody (OTI1G4), TrueMAB™

Product Details

CF811806

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1G4

Immunogen

Full length human recombinant protein of human ATP5I produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP synthase e chain, mitochondrial; ATP synthase F(0) complex subunit e, mitochondrial; ATP synthase membrane subunit e; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E; ATPase subunit e; F1F0-ATP synthase, murine e subunit

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Gene Aliases: ATP5I; ATP5K; ATP5ME

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UniProt ID: (Human) P56385

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Entrez Gene ID: (Human) 521

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Function(s)
hydrogen ion transmembrane transporter activity ATPase activity transmembrane transporter activity
Process(es)
ATP biosynthetic process mitochondrial ATP synthesis coupled proton transport
It has to be done as per old AB suggested Products section.
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