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  • Primary Antibodies ›
  • ATP Synthase beta Antibodies

OriGene

ATP5B Monoclonal Antibody (OTI4E5), TrueMAB™

View all (43) ATP Synthase beta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ATP5B Monoclonal Antibody (OTI4E5), TrueMAB™

  • Antibody Testing Data (5)
ATP5B Antibody in Immunocytochemistry (ICC/IF)
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ATP5B Antibody in Immunocytochemistry (ICC/IF)
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ATP5B Antibody (CF500833) in ICC/IF

Anti-ATP5B mouse monoclonal antibody (TA500833) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B(RC201638). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP5B Antibody in Immunocytochemistry (ICC/IF)
ATP5B Antibody in Western Blot (WB)
ATP5B Antibody in Western Blot (WB)
ATP5B Antibody in Flow Cytometry (Flow)
ATP5B Antibody in Flow Cytometry (Flow)
ATP5B Monoclonal Antibody (OTI4E5), TrueMAB™

Product Details

CF500833

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:100
-
Product Specifications

Species Reactivity

Dog, Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI4E5

Immunogen

Full length human recombinant protein of human ATP5B produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP synthase F(1) complex catalytic subunit beta, mitochondrial; ATP synthase F(1) complex subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; f1-ATPase beta; F1-ATPase beta-subunit; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

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Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271

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UniProt ID: (Human) P06576, (Dog) P99504, (Rat) P10719

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Entrez Gene ID: (Human) 506, (Dog) 403669, (Rat) 171374

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Function(s)
transporter activity protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism calcium ion binding lipoprotein particle receptor activity
Process(es)
angiogenesis osteoblast differentiation generation of precursor metabolites and energy lipid metabolic process ATP biosynthetic process negative regulation of cell adhesion involved in substrate-bound cell migration mitochondrion organization ATP hydrolysis coupled proton transport proton transport mitochondrial ATP synthesis coupled proton transport regulation of intracellular pH receptor-mediated endocytosis ATP synthesis coupled proton transport ATP metabolic process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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