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  • ATP5A1 Antibodies

Invitrogen

ATP5A1 Recombinant Rabbit Monoclonal Antibody (6M3B8)

View all (30) ATP5A1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite ATP5A1 Recombinant Rabbit Monoclonal Antibody (6M3B8)

  • Antibody Testing Data (6)
ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
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ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 6

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ATP5A1 Antibody (MA5-35082) in ICC/IF

Confocal imaging (Immunocytochemistry) of ATP5A1 in NIH/3T3 cells. Samples were incubated with ATP5A1 Monoclonal antibody (Product # MA5-35082) using a dilution of 1:100 (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody in Immunoprecipitation (IP)
ATP5A1 Recombinant Rabbit Monoclonal Antibody (6M3B8)

Product Details

MA5-35082

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:6,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000
-

Immunocytochemistry (ICC/IF)

1:200-1:2,000
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 400 µg-600 µg extracts of
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6M3B8

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATP5A1 (P25705)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848987

Product Specific Information

Immunogen sequence: VPIGRGQREL IIGDRQTGKT SIAIDTIINQ KRFNDGSDEK KKLYCIYVAI GQKRSTVAQL VKRLTDADAM KYTIVVSATA SDAAPLQYLA PYSGCSMGEY F

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP synthase alpha chain, mitochondrial; ATP synthase F(1) complex subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

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Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM

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UniProt ID: (Human) P25705, (Rat) P15999, (Mouse) Q03265

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Entrez Gene ID: (Human) 498, (Rat) 65262, (Mouse) 11946

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Function(s)
protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ADP binding nucleotide binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
Process(es)
negative regulation of endothelial cell proliferation lipid metabolic process ATP synthesis coupled proton transport ATP hydrolysis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport ATP biosynthetic process transport ion transport embryo development proton transport mitochondrial ATP synthesis coupled proton transport
It has to be done as per old AB suggested Products section.
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