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  • ATP5A1 Antibodies

Invitrogen

ATP5A1 Polyclonal Antibody

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1 Reference
View all (30) ATP5A1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ATP5A1 Polyclonal Antibody

  • Antibody Testing Data (12)
  • Advanced Verification (2)
ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
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ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
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ATP5A1 Antibody (PA5-27504) in ICC/IF

Immunofluorescence analysis of ATP5A was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with mtTFA Rabbit Polyclonal Antibody (Product # PA5-27504) at 1:150 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:200... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
ATP5A1 Antibody in Immunocytochemistry (ICC/IF)
ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP5A1 Antibody in Immunohistochemistry (Frozen) (IHC (F))
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody in Western Blot (WB)
ATP5A1 Antibody
ATP5A1 Antibody
ATP5A1 Polyclonal Antibody

Product Details

PA5-27504

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATP5A1. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544980

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, mouse brain, rat brain.

Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (90%), Xenopus laevis (89%), Dog (95%), Pig (96%), Chicken (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP synthase alpha chain, mitochondrial; ATP synthase F(1) complex subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

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Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM

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UniProt ID: (Human) P25705, (Rat) P15999, (Mouse) Q03265

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Entrez Gene ID: (Human) 498, (Rat) 65262, (Mouse) 11946

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Function(s)
protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ADP binding nucleotide binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
Process(es)
negative regulation of endothelial cell proliferation lipid metabolic process ATP synthesis coupled proton transport ATP hydrolysis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport ATP biosynthetic process transport ion transport embryo development proton transport mitochondrial ATP synthesis coupled proton transport
It has to be done as per old AB suggested Products section.
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