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  • ATP1A1 Antibodies

Invitrogen

ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor™ 555

1 Published Figure
1 Reference
View all (40) ATP1A1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor™ 555

  • Antibody Testing Data (6)
  • Published Figures (1)
ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
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ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 7

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ATP1A1 Antibody (MA3-928-A555) in ICC/IF

Immunofluorescent analysis of Na/K ATPase Alpha (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Na/K ATPase Alpha Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-928-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,0... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP1A1 Antibody in Flow Cytometry (Flow)
ATP1A1 Antibody in Immunohistochemistry (IHC)
ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor™ 555

Product Details

MA3-928-A555

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-10 µg/mL
-
Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Human, Mouse, Sheep, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M7-PB-E9

Immunogen

Purified alpha sodium/potassium ATPase from sheep kidney
View immunogen

Conjugate

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555
  • Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 647 
  • Request custom conjugation

Excitation/Emission Max

553/568 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2633349

Product Specific Information

MA3928A555 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.

The MA3928A555 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: (Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product

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Gene Aliases: ATP1A1; Atpa-1; BC010319; BOS_3049; Nkaa1b

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UniProt ID: (Sheep) P04074, (Human) P05023, (Pig) P05024, (Bovine) Q08DA1, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2

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Entrez Gene ID: (Dog) 403992, (Sheep) 443381, (Human) 476, (Pig) 397481, (Bovine) 282144, (Chicken) 396530, (Rat) 24211, (Mouse) 11928

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Function(s)
sodium:potassium-exchanging ATPase activity ATP binding metal ion binding protein binding phosphatase activity protein kinase binding protein domain specific binding ankyrin binding potassium ion binding sodium ion binding ADP binding phosphatidylinositol 3-kinase binding chaperone binding steroid hormone binding nucleotide binding hydrolase activity
Process(es)
regulation of sodium ion transport potassium ion transport sodium ion transport metabolic process establishment or maintenance of transmembrane electrochemical gradient ATP hydrolysis coupled proton transport regulation of the force of heart contraction cellular sodium ion homeostasis regulation of blood pressure potassium ion import energy coupled proton transmembrane transport, against electrochemical gradient dephosphorylation cellular potassium ion homeostasis negative regulation of glucocorticoid biosynthetic process sodium ion transmembrane transport sodium ion export from cell response to drug negative regulation of heart contraction positive regulation of heart contraction positive regulation of striated muscle contraction relaxation of cardiac muscle cardiac muscle contraction membrane hyperpolarization cellular response to mechanical stimulus cellular response to steroid hormone stimulus potassium ion transmembrane transport regulation of cardiac muscle cell contraction membrane repolarization response to glycoside ion transmembrane transport membrane repolarization during cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction regulation of cardiac conduction potassium ion import across plasma membrane transport ion transport ATP biosynthetic process
It has to be done as per old AB suggested Products section.
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