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  • ATP Synthase beta Antibodies

Invitrogen

ATP Synthase beta Recombinant Rabbit Monoclonal Antibody (JM10-90)

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View all (43) ATP Synthase beta antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite ATP Synthase beta Recombinant Rabbit Monoclonal Antibody (JM10-90)

  • Antibody Testing Data (14)
  • Advanced Verification (1)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
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ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
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ATP Synthase beta Antibody (MA5-32589) in ICC/IF

Immunofluorescence analysis of ATP5F1B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATP Synthase beta Recombinant Rabbit Monoclonal Antibody (JM10-90) (Product # MA5-32589) at 1:100 dilution in 0.1... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATP Synthase beta Antibody in Western Blot (WB)
ATP Synthase beta Antibody in Western Blot (WB)
ATP Synthase beta Antibody in Western Blot (WB)
ATP Synthase beta Antibody in Western Blot (WB)
ATP Synthase beta Antibody
ATP Synthase beta Recombinant Rabbit Monoclonal Antibody (JM10-90)

Product Details

MA5-32589

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Immunoprecipitation (IP)

1:10-1:50
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM10-90

Immunogen

Synthetic peptide within Human ATPB aa 141-184
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809866

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP synthase F(1) complex catalytic subunit beta, mitochondrial; ATP synthase F(1) complex subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

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Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; fj13e04; fj55c09; HEL-S-271; hm:zehn0534; im:6793121; wu:fj13e04; wu:fj38d01; wu:fj55c09; zgc:111961

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UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480

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Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947, (Zebrafish) 554135

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Function(s)
transporter activity protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism calcium ion binding lipoprotein particle receptor activity nucleotide binding hydrolase activity hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
Process(es)
angiogenesis osteoblast differentiation generation of precursor metabolites and energy lipid metabolic process ATP biosynthetic process negative regulation of cell adhesion involved in substrate-bound cell migration mitochondrion organization ATP hydrolysis coupled proton transport proton transport mitochondrial ATP synthesis coupled proton transport regulation of intracellular pH receptor-mediated endocytosis ATP synthesis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport transport ion transport
It has to be done as per old AB suggested Products section.
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