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  • ATG10 Antibodies

Osenses

ATG10 Polyclonal Antibody

2 References
View all (15) ATG10 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite ATG10 Polyclonal Antibody

  • Antibody Testing Data (7)
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ATG10 Antibody (OSA00016W-150UL) in IHC (P)

IHC-P on paraffin sections of human brain, hippocampus. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ATG10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Details

OSA00016W-150UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000
-

Miscellaneous PubMed (MISC)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from human autophagy-related protein 10 (ATG10, APG10) conjugated to immunogenic carrier protein has been used as the antigen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.

Specificity of this antibody: ATG10.

Target Information

ATG10 is involved in autophagy which is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12 -ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein ATG10 is ubiquitously expressed and the human APG10L gene is localized on chromosome 5q14.2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: APG10-like; ATG10 autophagy related 10 homolog; Autophagy-related protein 10; DKFZp586I0418; FLJ13954; Ubiquitin-like-conjugating enzyme ATG10

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Gene Aliases: APG10; APG10L; ATG10; PP12616

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UniProt ID: (Human) Q9H0Y0

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Entrez Gene ID: (Human) 83734

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Function(s)
ligase activity Atg12 transferase activity
Process(es)
protein lipidation autophagy protein transport macroautophagy positive regulation of protein modification process protein modification by small protein conjugation autophagy in response to ER overload
It has to be done as per old AB suggested Products section.
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