Invitrogen

APE1 Recombinant Rabbit Monoclonal Antibody (3W5N10)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
APE1 Recombinant Rabbit Monoclonal Antibody (3W5N10)

Product Details

MA5-35087

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3W5N10

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human APEX1/APE1 (P27695)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848992

Product Specific Information

Immunogen sequence: MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD QKTSPSGKPA TLKICSWNVD GLRAWIKKKG LDWVKEEAPD ILCLQETKCS

Target Information

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; protein REF-1; redox factor 1; Redox factor-1; REF-1

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Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1

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UniProt ID: (Human) P27695, (Mouse) P28352, (Rat) P43138

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Entrez Gene ID: (Human) 328, (Mouse) 11792, (Rat) 79116

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Function(s)
DNA binding damaged DNA binding double-stranded telomeric DNA binding transcription coactivator activity transcription corepressor activity DNA-(apurinic or apyrimidinic site) lyase activity endonuclease activity endodeoxyribonuclease activity RNA-DNA hybrid ribonuclease activity phosphodiesterase I activity uracil DNA N-glycosylase activity protein binding phosphoric diester hydrolase activity double-stranded DNA exodeoxyribonuclease activity double-stranded DNA 3'-5' exodeoxyribonuclease activity 3'-5' exonuclease activity oxidoreductase activity site-specific endodeoxyribonuclease activity, specific for altered base chromatin DNA binding protein complex binding poly(A) RNA binding metal ion binding NF-kappaB binding RNA binding nuclease activity endoribonuclease activity exonuclease activity hydrolase activity lyase activity phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity
Process(es)
DNA repair base-excision repair DNA recombination transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus aging negative regulation of smooth muscle cell migration regulation of mRNA stability cell redox homeostasis positive regulation of DNA repair oxidation-reduction process cellular response to hydrogen peroxide DNA demethylation nucleic acid phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of G1/S transition of mitotic cell cycle regulation of nucleic acid-templated transcription telomere maintenance base-excision repair, base-free sugar-phosphate removal response to drug cellular response to cAMP cellular response to peptide hormone stimulus negative regulation of nucleic acid-templated transcription DNA catabolic process, endonucleolytic DNA catabolic process, exonucleolytic response to organonitrogen compound cellular response to organonitrogen compound
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