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  • Primary Antibodies ›
  • ANP Antibodies

Invitrogen

ANP Polyclonal Antibody

View all (21) ANP antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ANP Polyclonal Antibody

  • Antibody Testing Data (4)
ANP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ANP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ANP Antibody (PA5-79759) in IHC (P)

Immunohistochemistry analysis of ANP on paraffin-embedded mouse cardiac muscle tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ANP polyclonal antibody (Product# PA5-79759) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Develop... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ANP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ANP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ANP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ANP Antibody in Western Blot (WB)
ANP Polyclonal Antibody

Product Details

PA5-79759

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived rat ANP recombinant protein (Position: N25-R122).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746874

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat heart tissue, mouse heart tissue. IHC: rat cardiac muscle tissue, mouse cardiac muscle tissue, rat small intestine tissue.

Target Information

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: atrial natriuretic factor; Atrial natriuretic factor prohormone; atrial natriuretic peptide; Atrial natriuretic peptide prohormone; Atriopeptigen; Cardiodilatin; CDD; Natriuretic peptide precursor A (pronatriodilatin, also Anf, Pnd); natriuretic peptide precursor A variant NPPA-M1; natriuretic peptide precursor A variant NPPA-M2; natriuretic peptide precursor A variant NPPA-M3; natriuretic peptide precursor type A; Natriuretic peptides A; preproANF; preproANP; preproCDD-ANF; prepronatriodilatin; Pronatriodilatin; urodilatin

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Gene Aliases: ANF; ANP; Nppa; Pnd; RATANF

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UniProt ID: (Rat) P01161, (Mouse) P05125

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Entrez Gene ID: (Rat) 24602, (Mouse) 230899

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Function(s)
receptor binding hormone activity neuropeptide hormone activity peptide hormone receptor binding neuropeptide receptor binding
Process(es)
response to hypoxia negative regulation of systemic arterial blood pressure cGMP biosynthetic process receptor guanylyl cyclase signaling pathway neuropeptide signaling pathway heart development female pregnancy regulation of blood pressure positive regulation of heart rate cardiac muscle hypertrophy in response to stress negative regulation of cell growth response to insulin regulation of body fluid levels regulation of blood vessel size multicellular organismal water homeostasis regulation of atrial cardiac muscle cell membrane repolarization positive regulation of cardiac muscle contraction cellular response to mechanical stimulus regulation of high voltage-gated calcium channel activity positive regulation of delayed rectifier potassium channel activity regulation of calcium ion transmembrane transport via high voltage-gated calcium channel positive regulation of potassium ion export across plasma membrane negative regulation of collecting lymphatic vessel constriction
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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