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ALPL Monoclonal Antibody (4H1)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite ALPL Monoclonal Antibody (4H1)

  • Antibody Testing Data (1)
ALPL Antibody in Western Blot (WB)
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ALPL Antibody in Western Blot (WB)
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ALPL Antibody (H00000249-M01) in WB

Western Blot analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1. Lane 1: ALPL transfected lysate(57.3 KDa). Lane 2: Non-transfected lysate. . {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ALPL Antibody in Western Blot (WB)
ALPL Monoclonal Antibody (4H1)

Product Details

H00000249-M01

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

ELISA (ELISA)

10 µg/mL
-

Functional Assay (Functional)

1-5 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

4H1

Immunogen

ALPL (AAH21289.3, 29 a.a. approximately 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Product may be used with Western Blot (Transfected lysate).

Immunogen sequence: WRDQAQETLK YALELQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNPGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERSR

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue-nonspecific ALP

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Gene Aliases: ALPL; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP

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UniProt ID: (Human) P05186

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Entrez Gene ID: (Human) 249

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Function(s)
alkaline phosphatase activity protein binding pyrophosphatase activity metal ion binding
Process(es)
skeletal system development osteoblast differentiation endochondral ossification developmental process involved in reproduction dephosphorylation response to lipopolysaccharide response to vitamin D response to antibiotic response to glucocorticoid cellular response to organic cyclic compound cementum mineralization
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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