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  • ALPL Antibodies

Invitrogen

ALPL Polyclonal Antibody

View all (56) ALPL antibodies

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Datasheet
Protocols
Questions & Answers

Cite ALPL Polyclonal Antibody

  • Antibody Testing Data (3)
ALPL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ALPL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ALPL Antibody (PA5-109383) in IHC (P)

Immunohistochemistry analysis of ALPL in paraffin-embedded human colon carcinoma. Samples were incubated with ALPL Polyclonal antibody (Product # PA5-109383) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ALPL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ALPL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ALPL Antibody in Western Blot (WB)
ALPL Polyclonal Antibody

Product Details

PA5-109383

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-260 of human Alkaline Phosphatase (ALPL) (NP_0004693)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.86 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854794

Product Specific Information

Immunogen sequence: GVKANEGTVG VSAATERSRC NTTQGNEVTS ILRWAKDAGK SVGIVTTTRV NHATPSAAYA HSADRDWYSD NEMPPEALSQ GCKDIAYQLM HNIRDIDVIM GGGRKYMYPK NKTDVEYESD EKARGTRLDG LDLVDTWKSF K

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alkaline phosphatase 2; alkaline phosphatase 2, liver; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNSALP

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Gene Aliases: Akp-2; Akp2; ALPL; AP-TNAP; APTNAP; HOPS; PHOA; TNAP; TNSALP

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UniProt ID: (Human) P05186, (Rat) P08289, (Mouse) P09242

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Entrez Gene ID: (Human) 249, (Rat) 25586, (Mouse) 11647

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Function(s)
alkaline phosphatase activity protein binding pyrophosphatase activity metal ion binding catalytic activity hydrolase activity phosphatase activity
Process(es)
osteoblast differentiation endochondral ossification developmental process involved in reproduction dephosphorylation response to lipopolysaccharide response to vitamin D response to antibiotic response to glucocorticoid cellular response to organic cyclic compound cementum mineralization metabolic process skeletal system development
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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