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  • ALOX5 Antibodies

Invitrogen

ALOX5 Polyclonal Antibody

View all (45) ALOX5 antibodies

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Datasheet
Protocols
Questions & Answers

Cite ALOX5 Polyclonal Antibody

  • Antibody Testing Data (3)
ALOX5 Antibody in Western Blot (WB)
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ALOX5 Antibody in Western Blot (WB)
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ALOX5 Antibody (PA1-46003) in WB

Western Blot was performed using Anti-ALOX5 Polyclonal Antibody (Product # PA1-46003) and a 70 kDa band corresponding to Arachidonate 5-lipoxygenase was observed in THP1 cells treated with both LPS and human TGF beta 1 and U2OS cells treated with Actinomycin D. Expression was also seen in Daudi, Raji and Raw 264.7 as compared to K-562, U-937, MOLT-4 and NIH/3T3 which are reported to be negative for ALOX5. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with Lipopolysaccharide (100 ng/mL) and human TGF beta 1 (1 ng/mL) f... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ALOX5 Antibody in Western Blot (WB)
ALOX5 Antibody in Western Blot (WB)
ALOX5 Antibody in Western Blot (WB)
ALOX5 Polyclonal Antibody

Product Details

PA1-46003

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to a C-terminal region within residues 600-674 of human 5-Lipoxygenase.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_906415

Product Specific Information

Suggested positive control: human Peripheral Blood Leukocyte protein.

Target Information

This gene encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5 - Lipoxygenase; 5-lipoxygenase; 5-LO; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; LOX-5; lox5; Polyunsaturated fatty acid 5-lipoxygenase; RP11-67C2.3

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Gene Aliases: 5-LO; 5-LOX; 5LO; 5LPG; 5LX; AI850497; ALOX5; BOS_24317; F730011J02; LOG5; LOX5A

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UniProt ID: (Human) P09917, (Rat) P12527, (Mouse) P48999

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Entrez Gene ID: (Human) 240, (Bovine) 404074, (Rat) 25290, (Mouse) 11689, (Rabbit) 100341714

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Function(s)
arachidonate 5-lipoxygenase activity iron ion binding protein binding oxidoreductase activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen metal ion binding dioxygenase activity
Process(es)
acute inflammatory response leukotriene production involved in inflammatory response leukotriene metabolic process inflammatory response response to nutrient sensory perception of pain arachidonic acid metabolic process leukotriene biosynthetic process lipoxygenase pathway positive regulation of vasoconstriction response to hyperoxia oxidation-reduction process lipoxin metabolic process
It has to be done as per old AB suggested Products section.
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