Invitrogen

AIF Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
AIF Polyclonal Antibody

Product Details

PA5-96166

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_0041991)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.58 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807968

Product Specific Information

Immunogen sequence: FPEKGNMGKI LPEYLSNWTM EKVRREGVKV MPNAIVQSVG VSSGKLLIKL KDGRKVETDH IVAAVGLEPN VELAKTGGLE IDSDFGGFRV NAELQARSNI WVAGDAACFY DIKLGRRRVE HHDHAVVSGR LAGENMTGAA KPYWHQSMFW SDLGPDVGYE AIGLVDSSLP TVGVFAKATA QDNPKSATEQ SGTGIRSESE TESEASEITI PPSTPAVPQA PVQGEDYGKG VIFYLRDKVV VGIVLWNIFN RMPIARKIIK DGEQHEDLNE VAKLFNIHED; Positive Samples: SW620, 22Rv1, H460, PC-3, U-937, Mouse liver, Mouse kidney, Mouse heart; Cellular Location: Cytoplasm, Mitochondrion inner membrane, Mitochondrion intermembrane space, Nucleus, perinuclear region

Target Information

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AG1; AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; harlequin; hTLP19; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

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Gene Aliases: AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8

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UniProt ID: (Human) O95831, (Mouse) Q9Z0X1, (Rat) Q9JM53

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Entrez Gene ID: (Human) 9131, (Mouse) 26926, (Rat) 83533

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Function(s)
DNA binding protein binding electron carrier activity NAD(P)H oxidase activity oxidoreductase activity, acting on NAD(P)H protein dimerization activity FAD binding C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity aldo-keto reductase (NADP) activity electron-transferring-flavoprotein dehydrogenase activity isocitrate dehydrogenase activity mevaldate reductase activity gluconate dehydrogenase activity steroid dehydrogenase activity oxidoreductase activity epoxide dehydrogenase activity 5-exo-hydroxycamphor dehydrogenase activity 2-hydroxytetrahydrofuran dehydrogenase activity acetoin dehydrogenase activity phenylcoumaran benzylic ether reductase activity D-xylose:NADP reductase activity L-arabinose:NADP reductase activity D-arabinitol dehydrogenase, D-ribulose forming (NADP+) activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor steroid dehydrogenase activity, acting on the CH-CH group of donors (R)-(-)-1,2,3,4-tetrahydronaphthol dehydrogenase activity 3-hydroxymenthone dehydrogenase activity very long-chain-3-hydroxyacyl-CoA dehydrogenase activity dihydrotestosterone 17-beta-dehydrogenase activity (R)-2-hydroxyisocaproate dehydrogenase activity L-arabinose 1-dehydrogenase (NADP+) activity L-xylulose reductase (NAD+) activity 3-ketoglucose-reductase activity flavin adenine dinucleotide binding (R)-2-hydroxyglutarate dehydrogenase activity D-arabinitol dehydrogenase, D-xylulose forming (NADP+) activity
Process(es)
response to ischemia DNA catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process response to toxic substance chromosome condensation positive regulation of apoptotic process positive regulation of neuron apoptotic process cell redox homeostasis neuron apoptotic process oxidation-reduction process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to estradiol stimulus cellular response to nitric oxide cellular response to oxygen-glucose deprivation response to L-glutamate regulation of apoptotic DNA fragmentation cellular response to aldosterone response to oxidative stress apoptotic mitochondrial changes positive regulation of cell death neuron differentiation mitochondrial respiratory chain complex I assembly
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