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  • HTR7 Antibodies

Alomone Labs, LTD

5HT7 Receptor/HTR7 (extracellular) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite 5HT7 Receptor/HTR7 (extracellular) Polyclonal Antibody

  • Antibody Testing Data (5)
5HT7 Receptor/HTR7 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))
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5HT7 Receptor/HTR7 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))
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FIGURE: 1 / 5

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5HT7 Receptor/HTR7 (extracellular) Antibody (ASR-037-200UL) in IHC (F)

Expression of5HT7 Receptor in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, shows5HT7Rimmunoreactivity (green) in neuronal profiles in the hilus (vertical arrows) and in glial profiles in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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5HT7 Receptor/HTR7 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))
5HT7 Receptor/HTR7 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))
5HT7 Receptor/HTR7 (extracellular) Antibody in Western Blot (WB)
5HT7 Receptor/HTR7 (extracellular) Antibody in Western Blot (WB)
5HT7 Receptor/HTR7 (extracellular) Antibody in Flow Cytometry (Flow)
5HT7 Receptor/HTR7 (extracellular) Polyclonal Antibody

Product Details

ASR-037-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:300
-

Flow Cytometry (Flow)

5 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CGEQINYGRVEK, corresponding to amino acid residues 73-84 of rat 5-HT7, Extracellular, N-terminus
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

Target Information

5-HT7 Receptor is a Serotonin Receptor with several functions, including regulation of the circadian rhythm and mediation of smooth-muscle relaxation in the vasculature and GI tract. This receptor has been implicated in depression, autism, and other neuropsychiatric disorders. Three protein isoforms have been documented: isoform a: 445aa; isoform b: 432aa; isoform d: 479aa. 5-HT7 Receptor has been reported in aorta, brain, heart, placenta, small intestine, spleen, artery, and vessel. An EST has been isolated from a testis library.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5-HT-7; 5-HT-X; 5-HT7; 5-HT7 receptor; 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled); 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled; 5-hydroxytryptamine receptor 7; 5HT7 Receptor; GPRFO; high affinity serotonin receptor (5HT7); HT7; OTTHUMP00000020077; OTTHUMP00000020078; OTTHUMP00000020079; Serotonin receptor 7

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Gene Aliases: 5-HT7; 5Ht7; HTR7

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UniProt ID: (Human) P34969, (Mouse) P32304, (Rat) P32305

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Entrez Gene ID: (Human) 3363, (Mouse) 15566, (Rat) 65032

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Function(s)
G-protein coupled serotonin receptor activity signal transducer activity G-protein coupled receptor activity neurotransmitter receptor activity
Process(es)
smooth muscle contraction signal transduction G-protein coupled receptor signaling pathway adenylate cyclase-activating serotonin receptor signaling pathway serotonin receptor signaling pathway chemical synaptic transmission memory circadian rhythm positive regulation of neuron projection development negative regulation of serotonin secretion urinary bladder smooth muscle contraction vasoconstriction negative regulation of circadian sleep/wake cycle, REM sleep behavioral response to pain detection of mechanical stimulus involved in sensory perception of pain response to corticosterone micturition long-term synaptic potentiation dopaminergic neuron differentiation G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger response to electrical stimulus blood circulation
It has to be done as per old AB suggested Products section.
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